L’hybridation in situ couplée à la cytométrie en flux
Une méthode de détection rapide et fiable de Brettanomyces dans les vins
Virginie Serpaggi (1), Fabienne Remize (2), Anabelle Sequeira-Le Grand (3), Hervé Alexandre (1)
(1) Institut Universitaire de la Vigne et du Vin Jules Guyot - Université de Bourgogne - Dijon - France.
(2) CTCPA - Avignon - France.
(3) Plate-forme Technique de Cytométrie - Faculté de médecine - Université de Bourgogne - Dijon - France.
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Pradelles, R., Alexandre, H., Ortiz-Julien, A., Chassagne, D., 2008. Effect of yeast cell-wall characteristics on 4-ethylphenol sorption capacity in model wine. Journal of Agricultural and Food Chemistry 56 (24), 11854-11861.
Röder, C., Köning, H., Fröhlich, J., 2007. Species-specific identification of Dekkera/Brettanomyces yeasts by fluorescently labeled DNA probes targeting the 26S rRNA. FEMS Yeast Research 6, 1013-1026.
Salazar, O., Asenjo, J.A., 2007. Enzymatic lysis of microbial cells. Biotechnology Letters 29, 985-994.
Silva, P., Cardoso, H., Geros, H., 2004. Studies on the wine spoilage capacity of Brettanomyces/Dekkera spp. American Journal of Enology and Viticulture 55, 1, 65-72.
Stender, H., Kurtzman, C., Hyldig?Nielsen, J.J., Sorensen, D., Broomer, A., Oliviera, K., Perry O’Keefe, H., Sage, A., Young, B., Coull, J., 2001. Identification of Dekkera bruxellensis (Brettanomyces) from wine by Fluorescence In Situ Hybridization using peptide nucleic acid probes. Applied Environmental Microbiology 67, 938-941.
Tessonnière, H., Vidal, S., Barnavon, L., Alexandre, H., Remize, F., 2009. Design and performance testing of a real-time PCR assay for sensitive and reliable direct quantification of Brettanomyces in wine. International Journal of Food Microbiology 129, 237-243.
Thornton, R., Godfrey, W., Gilmour, L., Alsharif, R., 2002. Evaluation of yeasts viability and concentration during wine fermentation usinf flow cytometry. Becton Dickinson Application Notes; http://www.bdbiosciences.com/immunocytometry_systems/application_notes/.
Vasserot, Y., Caillet, S., Maujean, A., 1997. Study of Anthocyanin Adsorption by Yeast Lees. Effect of Some Physicochemical Parameters. American Journal of Enology and Viticulture 48, 433-437.
Wagner, M., Horn, M., Daims, H., 2003. Fluorescence in situ hybridization for the identification and characterization of prokaryotes. Current Opinion in Microbiology 6, 302-309.
Mots Clefs : Cytométrie de flux Brettanomyce Vin rouge phénol volatil Fluorescence Levure Cheval Goût de souris
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